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ISRIB Integrated Stress Response Inhibitor

Name: ISRIB
Brand: Selleck Chemicals
SKU: S0706
Rating: 5 (26 reviews)

Cat.No.: S0706

ISRIB is an integrated stress response (ISR) inhibitor that potently reverses the effects of eukaryotic initiation factor 2α (eIF2α) phosphorylation.

Chemical Structure

Molecular Weight: 451.34

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Quality Control

Batch: Purity: 98.81%

98.81

Related Targets	CDK HSP PD-1/PD-L1 ROCK Wee1 DNA/RNA Synthesis Microtubule Associated Ras KRas Aurora Kinase
Other eIF Inhibitors	Zotatifin (eFT226) Salubrinal 4EGI-1 4E1RCat SBI-0640756 Briciclib

Solubility in DMSO or water

In vitro
Batch:

DMSO : 10 mg/mL (22.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 23 mg/mL (50.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 8 mg/mL (17.72 mM) Warmed with 60°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.600mg/ml (1.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.600mg/ml (1.33mM)
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	10%DMSO 1 90%PEG300 Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.11mM)	Taking the 1 mL working solution as an example, add 100 μL of 5 mg/ml clear DMSO stock solution to 900 μL of PEG300 and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Search by Cell Line | Choose a Research Area to Find Working Concentrations

ISRIB is a potent inhibitor of eIF2B (5 nM), eIF2α-P-dependent downstream signaling (5 nM), EIF2S1, ATF4. ISRIB exhibits the greatest inhibitory potency toward eIF2B (IC50 = 5 nM).

Selectivity & Specificity Details

Mechanism of Action

Information	ISRIB is a potent eIF2B activator and integrated stress response (ISR) inhibitor. It affects PERK/eIF2α/ATF4 signaling by promoting eIF2B decamer assembly and blocking eIF2α-P-dependent downstream signaling, effectively restoring protein synthesis, suppressing stress granule formation, and mitigating ER stress-induced apoptosis.
Read more about ISRIB IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	451.34	Formula	C₂₂H₂₄Cl₂N₂O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	548470-11-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	ClC1=CC=C(OCC(=O)NC2CCC(CC2)NC(=O)COC3=CC=C(Cl)C=C3)C=C1

C1=C0/X	C1:	LOG(C1):
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C8=C7/X	C8:	LOG(C8):

ISRIB Integrated Stress Response Inhibitor

Molecular Weight: 451.34

Quality Control

Solubility in DMSO or water

Molarity Calculator

In vivo Formulation Calculator (Clear solution)

Working concentrations for cell culture treatment

Search by Cell Line | Choose a Research Area to Find Working Concentrations

Selectivity & Specificity

Mechanism of Action

Chemical Information, Storage & Stability